Abstract

This disclosure presents the establishment of a new cell line from Pseudaletia unipuncta embryos. This cell line demonstrated the ability to produce high numbers of baculoviruses in cell culture. These virus particles are found internally in the cells in occlusion bodies. In the study of Pseudaletia unipuncta two baculoviruses were found to infect this species: P. unipuncta nuclear polyhedrosis virus (PuNPV), and P. unipuncta granulosis virus (PuGV). In addition, the cell line was also selected and cultured for its ability to grow in suspension while maintaining high levels of OB production.